PAF Acetylhydrolase Inhibitor Screening Assay Kit Reference: 10004380-96 Cayman’s PAF-AH Inhibitor Screening Assay uses 2-thio PAF as a substrate for PAF-AH. Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using DTNB also known as Ellman's reagent. Cayman's PAF-AH Inhibitor Screening assay includes human plasma PAF-AH and is a time-saving tool for screening inhibitors.
sPLA2 (Type V) Inhibitor Screening Assay Kit Reference: 10004883-96 The sPLA2 (Type V) Inhibitor Screening Assay is a convenient colorimetric assay designed for rapid screening of Type V sPLA2 inhibitors in a 96-well format. Each kit contains assay buffer, DTNB, diheptanoyl thio-PC, human sPLA2 (Type V), a 96-well plate, plate cover, and complete instructions.
Protein Carbonyl Colorimetric Assay Kit Reference: 10005020-96 Cayman’s Protein Carbonyl Colorimetric Assay is a colorimetric assay for the measurement of oxidized proteins. The carbonyls of protein samples are derivatized using 2,4-dinitrophenylhydrazine. Formation of a Schiff base produces the corresponding hydrazone which can be analyzed spectrophotometrically at 360-385 nm. This assay can be used to measure oxidized protein in plasma, serum, cell lysates, and tissue homogenates.
Fatty Acid Amide Hydrolase Inhibitor Screening Assay Kit Reference: 10005196-96 FAAH is a cytosolic serine hydrolase responsible for the degradation of fatty acid amides, including AEA. Finding inhibitors to FAAH could offer a beneficial approach toward the treatment of pain, obesity, and various neurological diseases where higher endocannabinoid activity would be beneficial. Cayman’s FAAH Inhibitor Screening Assay provides a convenient fluorescence-based method for screening FAAH inhibitors. FAAH hydrolyzes AMC-arachidonoyl amide resulting in the release of the fluorescent product, 7-amino-4-methylcoumarin (AMC). The fluorophore can be easily analyzed using an excitation wavelength between 340-360 nm and an emission wavelength between 450-465 nm.
Atherosclerosis Product Pack Reference: 10005292-1 Contains SOAT-1/ACAT-1 polyclonal antibody and blocking peptide, cholesteryl linoleate hydroperoxides, POV-PC, and PGPC
Renin Inhibitor Screening Assay Kit Reference: 10006270-96 Cayman’s Renin Inhibitor Screening Assay provides a convenient assay in a 96-well format for evaluating human renin inhibitors. The assay utilizes a renin-based synthetic peptide substrate which incorporates the fluorophore EDANS at one end and an EDAN-quenching molecule (Dabcyl) at the other end. After cleavage by renin, the peptide-EDANS product is released yielding bright fluorescence which can be easily analyzed using excitation wavelengths between 335-345 nm and emission wavelengths between 485-510 nm.
Myeloperoxidase Chlorination Fluorometric Assay Kit Reference: 10006438-2 Cayman’s Myeloperoxidase Chlorination Fluorometric Assay provides a convenient fluorescence-based method for detecting the MPO chlorination activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the non-fluorescent 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]-benzoic acid (APF), which is selectively cleaved by hypochlorite (-OCl) to yield the highly fluorescent compound fluorescein.(11242) Fluorescein fluorescence is analyzed with an excitation wavelength of 480-495 nm and an emission wavelength of 515-525 nm. The kit includes a MPO-specific inhibitor for distinguishing MPO activity from MPO-independent fluorescence.
HAT Inhibitor Screening Assay Kit Reference: 10006515-96 Cayman’s HAT Inhibitor Screening Assay provides a fast, fluorescence-based method for evaluating PCAF HAT inhibitors. The procedure requires only three easy steps, all performed in the same microplate. In the first step of the protocol, HAT is incubated with acetyl-CoA and the histone H3 peptide. During this time, HAT catalyzes the enzymatic transfer of acetyl groups from acetyl-CoA to the H3 peptide producing an acetylated peptide and CoASH. Following addition of isopropanol to stop the enzymatic reaction, CPM is added to the wells of the plate. CPM reacts with the free thiol groups present on CoASH forming a highly fluorescent product that is detected using excitation and emission wavelengths of 360-390 and 450-470 nm, respectively.
S-Nitrosylated Protein Detection Kit (Biotin Switch) Reference: 10006518-1 Cayman’s S-Nitrosylated Protein Detection Kit (Biotin Switch) employs a modification of the Jaffrey et al. 'Biotin-switch' method to allow for the direct visualization of S-nitrosylated proteins in whole cells or tissues, as well as by WB analysis. Using this method, free SH groups are first blocked (an addition of 125.1 amu per residue) and any S-NO bonds present in the sample are then cleaved. Biotinylation of the newly formed SH groups (an addition of 523.6 amu per residue) provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection.
PPARγ Transcription Factor Assay Kit Reference: 10006855-96 PPARs are ligand-activated transcription factors belonging to the large superfamily of nuclear receptors. PPARα primarily activates genes encoding proteins involved in fatty acid oxidation, while PPARγ primarily activates genes directly involved in lipogenic pathway and insulin signalling. Members of the PPAR family are important direct targets of many antidiabetic and hypolipidemic drugs. Cayman’s PPAR Transcription Factor Assays are a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.