Magnesium Colorimetric Assay Kit (Xylidyl Blue-I Method) Reference: JAI-CMG-035 Magnesium (Mg2+) is one of the most abundant trace metals in living organisms, contributing to a variety of biological functions including ATP and nucleic acid processing, energy metabolism, and enzymatic function. Magnesium forms complexes with multiple molecules such as phospholipids and ATP. Approximately 69% of magnesium ions are stored in bone. The rest are part of the intermediary metabolism about 70% being present in free form while the other 30% is bound to proteins (especially albumin), citrates, phosphate, and other complex formers. The Mg2+ serum level is kept constant within very narrow limits. Regulation takes place mainly via the kidneys, especially via the ascending loop of Henle. Low serum levels of magnesium have been associated with metabolic syndrome, diabetes mellitus type 2, and hypertension.
Zinc Colorimetric Assay Kit (5-Br-PAPS Method) Reference: JAI-CZN-001 Zinc (Zn2+) is an essential trace element, vital for the health of animals, plants, and microorganisms . Zinc is found in hundreds of enzymes, is stored and transferred in metallothioneins, and serves as structural ions in transcription factors. Zinc is often coordinated to the sides chains of amino acids, such as aspartic acid, glutamic acid, histidine and cysteine and is involved in nucleid acid and protein synthesis. It is therefore a necessary complement for cell replication. In humans, zinc interacts with a variety of organic ligands, and has roles in nucleic acid metabolism, apoptosis, neurological development, signal transduction, and gene expression. Zinc deficiency is related to skin lesions, irritability, loss of hair, growth retardation and impaired immunological functions.
Total Antioxidant Capacity (PAO) Test Kit Reference: JAI-KPA-050 Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention.
MDM2 E3 Ligase TR-FRET Kit Reference: SBB-KF0030 South Bay Bio’s MDM2 TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto GST-MDM2 in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto MDM2 in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
ITCH E3 Ligase TR-FRET Kit Reference: SBB-KF0035 South Bay Bio’s ITCH TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto ITCH in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto ITCH in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
Parkin E3 Ligase TR-FRET Kit Reference: SBB-KF0036 South Bay Bio’s Parkin TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto both wild-type Parkin and a more active mutant W403A in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto Parkin in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
Nedd4 E3 Ligase TR-FRET Kit Reference: SBB-KF0056 South Bay Bio’s NEDD4 E3 Ligase TR-FRET Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto NEDD4 in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto NEDD4 in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
XIAP E3 Ligase TR-FRET Kit Reference: SBB-KF0057 South Bay Bio’s XIAP E3 Ligase TR-FRET Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto XIAP in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto XIAP in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
Cullin4a/Rbx1/DDB1/CRBN E3 Ligase Complex TR-FRET Kit Reference: SBB-KF0120 South Bay Bio’s Cullin4a/Rbx1/DDB1/CRBN Neddylated E3 Ligase TR-FRET Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto the E3 complex in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto CRBN (or substrates) in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
20S Immunoproteasome Assay Kit Reference: SBB-KP0037 South Bay Bio's kit is designed to test for specific activity of 20S immunoproteasome. The kit provides purified 20S immunoproteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC) and Caspase-like activity of the immunoproteasome subunits beta1i/ PSMB9 (PAL-AMC) and beta5i/PSMB8 (ANW-AMC). Additionally, the compound, ONX-0914, which can be used to inhibit specifically the subunit beta5i/LMP7 20S immunoproteasome is included. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission= 445nm; allowing for a real-time read out of 20S immunoproteasome specific activity.
20S Constitutive Proteasome Assay Kit Reference: SBB-KP0038 South Bay Bio's kit is designed to test for specific activity of 20S proteasome. The kit provides purified 20S proteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC), Caspase-like activity of the proteasome subunits beta1/PSMB6 (LLE-AMC) and Chymotrypsin-like activity of beta5/PSMB5 (WLA-AMC). Additionally, the compound MG-132 is included, which can be used to inhibit the proteasome. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission=445nm; allowing for a real-time read out of 20S proteasome specific activity.
SARS-CoV-2 Inhibitor Screening Kit Reference: AG-48B-0001 Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome beta-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19). SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells. The SARS-CoV-2 Inhibitor Screening Kit contains key reagents required to facilitate identification of SARS-CoV-2 inhibitors. This inhibitor screen is based on a colorimetric ELISA kit, which measures the binding of the RBD of the Spike S protein from SARS-CoV-2 to its human receptor ACE2. This assay allows to identify and characterize the effect of different inhibitory molecules including antibodies or chemicals on the prevention of binding of SARS-CoV-2 virus to any ACE2-expressing cells.