CheKine™ Reducing Sugar(RS) Assay Kit（Colorimetric） Reference: KTB1360_96T CheKine™ Reduction Sugar (RS) Assay kit (colorimetric) provides a simple, convenient, rapid and colorimetric reducing sugar detection method. This kit can detect the reduction sugars concentration from liquid samples, such as animal and plant tissues homogenate, bacteria, cells and serum (plasma). The principle of kit is that 3,5-dinitrosalicylic acid can be reduced to brown-red amino compounds by reducing sugar in alkaline solution. There is a characteristic absorption peak 540nm. In a certain concentration range, the reducing sugar content is linearly related to the absorbance of 540nm. According to the standard curve, the reducing sugar content in the sample can be calculated.
CheKine™ Ceruloplasmin Assay Kit (Colorimetric) Reference: KTB1550_96T CheKine™ Ceruloplasmin Assay Kit (Colorimetric) provides a simple, convenient, rapid and colorimetric glycogen detection method. The principle of kit is Ceruloplasmin catalyzes the formation of colored substances from o-dianisidine dihydrochloride, with a characteristic absorption peak at 460 nm. According the enzyme activity definition, the ceruloplasmin activity in the sample can be calculated.
CheKine™ Advanced Oxidation Protein Products (AOPP) Assay Kit (Colorimetric) Reference: KTB1060_100T CheKine™ Advanced Oxidation Protein Products (AOPP) Assay Kit (Colorimetric) provides a simple, convenient, rapid and colorimetric total carbohydrate detection method. The principle of kit is the sample or standard reacts with the initiator and stop solution, and the product has an absorption peak at 340 nm. The content of AOPP in the sample is determined by detecting the OD value at 340 nm and comparing it with the standard curve.
CheKine™ Hydroxyl Free Radical Scavenging Capacity Assay Kit (Colorimetric)... Reference: KTB1091_48T Abbkine CheKine™ Hydroxyl Free Radical Scavenging Capacity Assay Kit (Colorimetric) (Salicylic Acid Method) is specially developed for the detection of Hydroxyl Free Radical Scavenging Capacity in various sample. The operation is simple and convenient, and the detection is more sensitive and accurate. In this assay, H2O2/ Fe2+ generates hydroxyl free radical through the Fenton reaction. Salicylic acid can effectively capture the generated hydroxyl free radical and reacts with them to produce 2,3- dihydroxybenzoic acid with a maximum absorption peak at 520 nm. After the substances with the capacity to scavenge hydroxyl free radical, resulting in the decrease of 520 nm absorbance. The value of 520 nm absorbance can reflects the Hydroxyl Free Radical Scavenging Capacity of the sample.
CheKine™ Hydroxyl Free Radical Scavenging Capacity Assay Kit (Colorimetric)... Reference: KTB1091_96T Abbkine CheKine™ Hydroxyl Free Radical Scavenging Capacity Assay Kit (Colorimetric) (Salicylic Acid Method) is specially developed for the detection of Hydroxyl Free Radical Scavenging Capacity in various sample. The operation is simple and convenient, and the detection is more sensitive and accurate. In this assay, H2O2/ Fe2+ generates hydroxyl free radical through the Fenton reaction. Salicylic acid can effectively capture the generated hydroxyl free radical and reacts with them to produce 2,3- dihydroxybenzoic acid with a maximum absorption peak at 520 nm. After the substances with the capacity to scavenge hydroxyl free radical, resulting in the decrease of 520 nm absorbance. The value of 520 nm absorbance can reflects the Hydroxyl Free Radical Scavenging Capacity of the sample.
CheKine™ Micro Hexokinase (HK) Activity Colorimetric Assay Kit Reference: KTB1123_48T CheKine™ Micro Hexokinase (HK) Activity Colorimetric Assay Kit provides a simple, convenient, rapid and colorimetric HK detection method.HK catalyzes glucose synthesis of 6-phosphoglucose, and 6-phosphoglucose dehydrogenase further catalyzes 6-phosphoglucose dehydrogenation to NADH, which reduces WST-8 to orange formazan, with the maximum absorption peak detected at around 450 nm. The enzyme activity was calculated by detecting the increase rate of light absorption at 450 nm
CheKine™ Micro Hexokinase (HK) Activity Colorimetric Assay Kit Reference: KTB1123_96T CheKine™ Micro Hexokinase (HK) Activity Colorimetric Assay Kit provides a simple, convenient, rapid and colorimetric HK detection method.HK catalyzes glucose synthesis of 6-phosphoglucose, and 6-phosphoglucose dehydrogenase further catalyzes 6-phosphoglucose dehydrogenation to NADH, which reduces WST-8 to orange formazan, with the maximum absorption peak detected at around 450 nm. The enzyme activity was calculated by detecting the increase rate of light absorption at 450 nm
CheKine™ 6-Phosphofructokinase(PFK) Activity Colorimetric Assay Kit Reference: KTB1124_48T CheKine™ 6-Phosphofructokinase(PFK) Activity Colorimetric Assay Kit provides a simple, convenient, rapid and colorimetric PFK detection method. PFK catalyzed fructosetose 6-phosphate and ATP to generate fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyzed NADH oxidation to NAD+ in turn. The NADH decline rate was measured at 340nm, which reflected the PFK activity
CheKine™ 6-Phosphofructokinase(PFK) Activity Colorimetric Assay Kit Reference: KTB1124_96T CheKine™ 6-Phosphofructokinase(PFK) Activity Colorimetric Assay Kit provides a simple, convenient, rapid and colorimetric PFK detection method. PFK catalyzed fructosetose 6-phosphate and ATP to generate fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyzed NADH oxidation to NAD+ in turn. The NADH decline rate was measured at 340nm, which reflected the PFK activity
CheKine™ Amino Acid (AA) Colorimetric Assay Kit Reference: KTB1460_96T The kit provides a simple method for detecting FC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria.
Cytoplasmic and Nuclear Protein Extraction Kit Reference: AR0106 Cytoplasmic and Nuclear Extraction Kit, For protein extraction in WB
Cell Counting Kit-8 (CCK-8) Reference: AR1160 Cell Counting Kit-8 (CCK-8), For quantitation of viable cell number in proliferation and cytotoxicity assays