Adipogenesis Assay Kit Reference: 10006908-1 Cayman’s Adipogenesis Assay provides the reagents required for studying the induction and inhibition of adipogenesis in the established 3T3-L1 model, using the adipogenesis induction procedure. This kit can also be used to screen drug candidates involved in adipogenesis. The classic Oil Red O staining for lipid droplets is used in this kit as an indicator of the degree of adipogenesis, and can be quantified with a plate reader after the dye is conveniently extracted from the lipid droplet.
HIF-1α Transcription Factor Assay Kit Reference: 10006910-96 The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis. HIF-1α has emerged as an important drug target in breast and prostate cancer, cardiovascular disease, and ischemia. Cayman’s HIF-1αTranscription Factor Assay is a sensitive ELISA-based method for detecting HIF-1α DNA binding activity in nuclear extracts and whole cell lysates.
NF-κB (human p50) Transcription Factor Assay Kit Reference: 10006912-96 The NF-κB/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), and c-Rel/NF-κB. Acting as dimers, these transcription factors bind to κB sites on DNA, thereby regulating expression of target genes. The most common Rel/NF-κB dimer in mammals contains p50-RelA (p50/p65) heterodimers and is an attractive target for potential therapeutics in human inflammation and certain other diseases. Cayman’s NF-κB (human p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting human NF-κB (p50) DNA binding activity in nuclear extracts and whole cell lysates. It will not cross-react with NF-κB (p65).
PPARδ Transcription Factor Assay Kit Reference: 10006914-96 PPARs are ligand-activated transcription factors belonging to the large superfamily of nuclear receptors. PPARα primarily activates genes encoding proteins involved in fatty acid oxidation, while PPARγ primarily activates genes directly involved in lipogenic pathway and insulin signaling. Members of the PPAR family are important direct targets of many antidiabetic and hypolipidemic drugs. Cayman’s PPAR Transcription Factor Assays are a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
PPARα Transcription Factor Assay Kit Reference: 10006915-96 PPARs are ligand-activated transcription factors belonging to the large superfamily of nuclear receptors. PPARα primarily activates genes encoding proteins involved in fatty acid oxidation, while PPARγ primarily activates genes directly involved in lipogenic pathway and insulin signalling. Members of the PPAR family are important direct targets of many antidiabetic and hypolipidemic drugs. Cayman’s PPAR Transcription Factor Assays are a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
Cholesterol Fluorometric Assay Kit Reference: 10007640-96 Cholesterol, particularly in the form of LDLs, is well understood to be associated with increased risk of coronary heart disease. The measurement of cholesterol is one of the most common tests performed in the clinical laboratory setting. However, simple and easy assays for cholesterol in the research lab have not been readily available. Cayman’s Cholesterol Assay provides a simple fluorometric method for the sensitive quantitation of total cholesterol in plasma or serum.
Cholesterol Fluorometric Assay Kit Reference: 10007640-480 Cholesterol, particularly in the form of LDLs, is well understood to be associated with increased risk of coronary heart disease. The measurement of cholesterol is one of the most common tests performed in the clinical laboratory setting. However, simple and easy assays for cholesterol in the research lab have not been readily available. Cayman’s Cholesterol Assay provides a simple fluorometric method for the sensitive quantitation of total cholesterol in plasma or serum.
PPARγ Ligand Screening Assay Kit Reference: 10007685-384 Cayman’s PPARγ FP-Based Ligand Screening Assay - Green provides a convenient fluorescence polarization (FP)-based single step assay for screening PPARγ ligands. In this assay, a ligand of PPARγ was conjugated to FITC and is used as the displacement probe. Agonists and antagonists of PPARγ will displace the fluorescent probe leading to a decrease in FP. The PPARγ FP-Based Ligand Screening Assay is a robust assay with a Z' of 0.81 and has a dynamic range of greater than 120 mP units. The assay has been validated using known agonists/ligands of PPARγ (Arachidonic Acid, Rosiglitazone, Troglitazone, etc.) with IC50 values ranging from nanomolar to millimolar concentrations.
SREBP-2 Transcription Factor Assay Kit Reference: 10007819-96 SREBP-2 is a transcription factor that performs a critical role in the transcriptional regulation of genes involved in cholesterol synthesis and uptake including HMG-CoA synthase, HMG-CoA reductase, and the LDL receptor.
NF-κB (p65) Transcription Factor Assay Kit Reference: 10007889-96 The NF-κB/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), and c-Rel/NF-κB. Acting as dimers, these transcription factors bind to κB sites on DNA, thereby regulating expression of target genes. The most common Rel/NF-κB dimer in mammals contains p50-RelA (p50/p65) heterodimers and is an attractive target for potential therapeutics in human inflammation and certain other diseases. Cayman’s NF-κB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting human NF-κB (p65) DNA binding activity in nuclear extracts and whole cell lysates. It will not cross-react with NF-κB (p50).
Thioredoxin Reductase Colorimetric Assay Kit Reference: 10007892-96 Cayman’s TrxR Assay provides a convenient method for quantifying mammalian TrxR activity from tissue homogenates and cell lysates in a colorimetric 96-well plate format. In this assay, TrxR uses NADPH to reduce DTNB to 5-thio-2-nitrobenzoic acid (TNB) which absorbs strongly at 405-414 nm. Measurement of TrxR activity in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction.
20S Proteasome Assay Kit Reference: 10008041-1 The 20S proteasome is the proteolytic core of a large protein degradation complex, the 26S proteasome. Proteasome inhibitors exhibit anti-inflammatory and antiproliferative effects, evidence that the proteasome may be an important drug target for the treatment for cancer and inflammatory diseases. Cayman’s 20S Proteasome Assay employs a specific 20S substrate, SUC-LLVY-AMC which, upon cleavage by the active enzyme, generates a highly fluorescent product with an emission wavelength at 480 nm.