KRISHZYME™ Nadroparin Factor IIa Assay Kit Reference: KBBA03N Nadroparin Factor lla is a chromogenic assay intended for the quantitative determination of Nadroparin in purified&solutions by measurement of factor IIa inhibition activity.
KRISHZYME™ Nadroparin Factor Xa Assay Kit Reference: KBBA04N Nadroparin Factor Xa is a chromogenic assay intended for the quantitative determination of Nadroparin in purified&solutions by measurement of factor Xa inhibition activity.
KRISHZYME™ Tinzaparin Factor IIa Assay Kit Reference: KBBA03T Tinzaparin Factor lla is a chromogenic assay intended for the quantitative determination of Tinzaparin in purifiedsolutions by measurement of factor IIa inhibition activity.
KRISHZYME™ Tinzaparin Factor Xa Assay Kit Reference: KBBA04T Tinzaparin Factor Xa is a chromogenic assay intended for the quantitative determination of Tinzaparin in purified solutions by measurement of factor Xa inhibition activity.
KRISHZYME™ Hyaluronidase Enzymatic Assay Kit Reference: KBBA05 Enzymatic Assay for Quantification of Hyaluronidase activity in cell culture supernatant and pharmaceutical preparations Hyaluronidase is a family of enzymes that degrade hyaluronic acid by catalyzing the hydrolysis of hyaluronan, a constituent of the interstitial barrier. Hyaluronidase lowers the viscosity of hyaluronan, thereby increasing tissue permeability. It is, therefore, used in medicine in conjunction with other drugs to speed their dispersion and delivery. About the kit: Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity, Recovery rates are between 85 - 115%, Ready to use with a standard protocol with break-apart pre-coated wells, Validated as per US FDA guidelines for Bioassays, Optimized for matrix effects to ensure higher sensitivity, Shelf life: 1 year. The KRISHZYME™ Hyaluronidase Assay kit uses a two-step turbidimetric reaction to measure hyaluronidase activity by the amount of hyaluronic acid that is hydrolyzed. A stop reagent halts the enzymatic reaction and forms turbidity with any residual hyaluronic acid in the well. The decrease in turbidity at 600 nm is directly proportional to hyaluronidase activity in the sample.
KRISHZYME™ Prekallikrein Activator Assay Kit Reference: KBBA30EP KRISHZYME Plasma Kallikrein Activitor Assay Kit utilizes the ability of active plasma kallikrein to cleave a synthetic pNA-based peptide substrate to release pNA, which can be easily quantified using a microplate reader. The kit is easy-to-use and can detect PK activity of purified plasma kallikrein and plasma samples according to the procedure recommended by the European Pharmacopoeia.
KRIBIOLISA™ Human IgG4 Reference: KBI1007 Immunoassay for the quantification of Human IgG in cell culture and biological samples. About the kit: Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity, Recovery rates are between 85 - 115%, Ready to use with a standard protocol with break-apart pre-coated wells, Validated as per US FDA guidelines for Bioassays, Optimized for matrix effects to ensure higher sensitivity, Shelf life: 1 year
KRIBIOLISA™ E.coli Host Cell DNA Kit Reference: KBBR01 For the production of biotechnological products, expression of therapeutic proteins in E.coli cells is commonly used. Due to which there is a possibility of DNA contamination from the host cells in the products which are manufactured. However, the limit of DNA contamination from the cell lines has been set by regulatory agencies According to WHO the limit of DNA contamination should not exceed 10 ng/dose in the products manufactured. One of the most common method which is widely used for detection of host cell DNA contamination by biopharmaceutical manufacturers is the PicoGreenTM Dye based assay. The research and development department at Krishgen Biosystems has designed a kit to detect and quantify host cell DNA in the products manufactured by recombinant expression in E.coli cell lines based on PicoGreenTM Dye assay. About the kit: Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity, Recovery rates are between 85 - 115%, Ready to use with a standard protocol with break-apart pre-coated wells, Validated as per US FDA guidelines for Bioassays, Optimized for matrix effects to ensure higher sensitivity, Shelf life: 1 year. The Krishgen E.coli Host Cell DNA Kit is based on the DNA dye binding assay which utilizes PicoGreenTM dye. The DNA samples and the standards are reacted with PicoGreenTM dye, which is a DNA intercalator that binds strongly to the double stranded DNA. Upon binding to DNA, PicoGreenTM dye fluoresces with an excitation of 485 nm and emission of 525 nm. The intensity of the fluorescent signal is proportional to the quantity of DNA in the standard or the samples.
KRIBIOLISA™ CHO Host Cell DNA Kit Reference: KBBR02 Immunoassay for the measurement of HEK Host Cell Proteins. For the production of biotechnological products, expression of therapeutic proteins in CHO cells is commonly used. Due to which there is a possibility of DNA contamination from the host cells in the products which are manufactured. However, the limit of DNA contamination from the cell lines has been set by regulatory agencies According to WHO the limit of DNA contamination should not exceed 10ng/dose in the products manufactured. One of the most common method which is widely used for detection of host cell DNA contamination by biopharmaceutical manufacturers is the PicoGreenTM Dye based assay. The research and development department at Krishgen Biosystems has designed a kit to detect and quantify host cell DNA in the products manufactured by recombinant expression in CHO cell lines based on PicoGreenTM Dye assay. About the kit: Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity, Recovery rates are between 85 - 115%, Ready to use with a standard protocol with break-apart pre-coated wells, Validated as per US FDA guidelines for Bioassays, Optimized for matrix effects to ensure higher sensitivity, Shelf life: 1 year. The Krishgen CHO Host Cell DNA Kit is based on the DNA dye binding assay which utilizes PicoGreenTM dye. The DNA samples and the standards are reacted with PicoGreenTM dye, which is a DNA interculator that binds strongly to the double stranded DNA. Upon binding to DNA, PicoGreenTM dye fluoresces with an excitation of 485 nm and emission of 525 nm. The intensity of the fluorescent signal is proportional to the quantity of DNA in the standard or the samples.
KRIBIOLISA™ Insect Cell Host Cell DNA Kit Reference: KBBR03 For the production of biotechnological products, expression of therapeutic proteins in insect cells is commonly used. Due to which there is a possibility of DNA contamination from the host cells in the products which are manufactured. However, the limit of DNA contamination from the cell lines has been set by regulatory agencies According to WHO the limit of DNA contamination should not exceed 10 ng/dose in the products manufactured. One of the most common method which is widely used for detection of host cell DNA contamination by biopharmaceutical manufacturers is the PicoGreenTM Dye based assay. The research and development department at Krishgen Biosystems has designed a kit to detect and quantify host cell DNA in the products manufactured by recombinant expression in insect cell lines based on PicoGreenTM Dye assay. About the kit: Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity, Recovery rates are between 85 - 115%, Ready to use with a standard protocol with break-apart pre-coated wells, Validated as per US FDA guidelines for Bioassays, Optimized for matrix effects to ensure higher sensitivity, Shelf life: 1 year. The Krishgen Insect Cell Host Cell DNA Kit is based on the DNA dye binding assay which utilizes PicoGreenTM dye. The DNA samples and the standards are reacted with PicoGreenTM dye, which is a DNA intercalator that binds strongly to the double stranded DNA. Upon binding to DNA, PicoGreenTM dye fluoresces with an excitation of 485 nm and emission of 525 nm. The intensity of the fluorescent signal is proportional to the quantity of DNA in the standard or the samples.