Phospho-Sure RTD Neuronal Cell & Soft tissue extraction buffer-100 mL Reference: B-100-PS Detergents typically present in cell lysis buffers are thought to disrupt organelles and compartments and increase the exposure of soluble phospho-proteins to phosphatases and proteases thereby resulting in uncontrolled dephosphorylation and proteolysis. Phospho-Sure RTD Neuronal extraction buffer is optimized for the extraction of phosphorylated proteins from neuronal and other soft tissues types. The buffer extracts the phosphoproteins in a native state without the use of harsh detergents or oxidizers, and it is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent based extraction buffers.
Phospho-Sure RTD Neuronal Cell & Soft tissue extraction buffer-200 mL Reference: B-200-PS Detergents typically present in cell lysis buffers are thought to disrupt organelles and compartments and increase the exposure of soluble phospho-proteins to phosphatases and proteases thereby resulting in uncontrolled dephosphorylation and proteolysis. Phospho-Sure RTD Neuronal extraction buffer is optimized for the extraction of phosphorylated proteins from neuronal and other soft tissues types. The buffer extracts the phosphoproteins in a native state without the use of harsh detergents or oxidizers, and it is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent based extraction buffers.
Secretory IgA Blocker for BEK-2211 and similar ELISA assays-1250 µg Reference: BL-001-1250 BL-001-1250 contains a proprietary mixture of proteins and buffer reagents designed to reduce the sIgA cross-reactivity present in certain substrates such as milk.Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-001-1250:BEK-2211, Mature BDNF; Application: human milkOne vial of BL-001-1250 contains 1250 μg of proprietary immunoglobulins, which is sufficient as sample diluent additive for one 96-well plate.Other ELISA assays may also benefit from addition of blocker BL-001-1250, but require optimization of working concentration and assay validation for accurate results.
Heterophilic antibody blocker for ELISA assays utilizing mouse assay... Reference: BL-002-1250 BL-002-1250 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing mouse immunoreagents. One vial of BL-002-1250 contains 1250 μg IgG which is sufficient as sample diluent additive for one 96-well plate if used at 50 μg/mL. Optimal blocker concentration needs to be validated by the end-user for accurate results.
Heterophilic antibody blocker for BEK-2226 & BEK-2218 and similar ELISA... Reference: BL-003-1000 BL-003-1000 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing a combination of sheep and mouse immunoreagents. Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-003-1000:BEK-2226, Human proNGF; Application: Serum, Heparin-PlasmaBEK-2218, Human NT4/5; Application: Citrate-Plasma One vial of BL-003-1000 contains 1000 μg IgG which is sufficient as sample diluent additive for one 96-well plate.Other ELISA assays that use sheep and mouse assay antibodies may also benefit from addition of blocker BL-003-1000, but require optimization of working concentration and assay validation for accurate results.
Heterophilic antibody blocker for BEK-2217, BEK-2221, BEK-2237 & BEK-2240 and... Reference: BL-004-500 BL-004-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing sheep immunoreagents. Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-004-500:BEK-2217/2240, Human proBDNF; Application: Serum, Citrate-PlasmaBEK-2221, Human NT3; Application: Plasma (Citrate and EDTA)BEK-2237, Human proBDNF; Application: Serum, EDTA-PlasmaOne vial of BL-004-500 contains 500 μg IgG which is sufficient as sample diluent additive for one 96-well plate.Other ELISA assays that use sheep assay antibodies may also benefit from addition of blocker BL-004-500, but require optimization of working concentration and assay validation for accurate results.
Heterophilic antibody blocker for BEK-2212 and similar ELISA assays-500 µg Reference: BL-005-500 BL-005-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing mouse immunoreagents. Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-005-500:BEK-2212, Human NGF; Application: Citrate-PlasmaOne vial of BL-005-500 contains 500 μg IgG which is sufficient as sample diluent additive for one 96-well plate.Other ELISA assays that use mouse assay antibodies may also benefit from addition of blocker BL-005-500, but require optimization of working concentration and assay validation for accurate results.
Black-Gold II Myelin Ready-to-Dilute Staining Kit with Toluidine Blue O... Reference: TR-100-BG Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system. The Black Gold II Ready-to-Dilute (RTD) Staining Kit allows you to localise myelin, both individual fibres and tracts, along with the option of co-localising cell bodies via the Toluidine Blue counter stain. Black Gold II labelled myelinated fibres appear nearly black while the Toluidine Blue O labelled cellular Nissl bodies are blue under bright field illumination.Black Gold II can demonstrate and characterise specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, domoic acid, 3-nitropropionic acid, Fluoro-Gold and isoniazid. Black Gold II can also be combined with other histochemical markers including Nissl stains, retrogradely transported fluorescent tracers and fluorescent markers of neuronal degeneration. The advantages associated with the Black-Gold II include high resolution, high contrast, short histochemical processing time, versatility and consistent reproducibility.
Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating... Reference: TR-100-FJ The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.
Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating... Reference: TR-100-FJT The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.
Fluoro-Jade B (FJB) Powder for identifying Degenerating Neurons-30 mg Reference: TR-150-FJB The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. The fluorescent dye Fluoro-Jade B (FJB), like its more purified brother Fluoro-Jade C (FJC), is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration. Fluoro-Jade B differs from FJC in that it is a slightly less refined chemical formulation and thus it does not quite provide the same level of signal to noise or high resolution as FJC. Nonetheless FJB is still widely used and works very well as a marker of degenerating neurons and even glia (see Damjanac M et al., Brain Res. 2007;1128(1):40-9). FJB operates nearly identically in protocol to that of FJC, and Fluoro-Jade B is compatible with several other labeling procedures including immunofluorescent and fluorescent Nissl techniques. Fluoro-Jade B stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade B exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.Note: This product is equivalent to discontinued product AG310 from Merck-Millipore.
Fluoro-Jade C (FJC) Powder for identifying Degenerating Neurons-30 mg Reference: TR-160-FJC The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.Note: This product is equivalent to discontinued product AG325 from Merck-Millipore.