Visikol HISTO Starter Kit Reference: HSK-1 With the Visikol HISTO tissue clearing approach you are now able to rapidly and easily image tissues in 3D without damaging the underlying cellular morphology. The Visikol HISTO approach has been designed to pair with immunolabeling and is reversible so that 3D histology can be followed up with traditional 2D histology. The Visikol HISTO tissue clearing approach for 3D tissue cultures is comprised of one reagent called Visikol HISTO-M. In our Starter Kit we have combined 30 mL of Visikol HISTO-M with the four buffer solutions required to process 3D tissue cultures for immunolabeling. All you need to do is add your and nuclear stains. This kit comes with enough material to process three 96-well plates of 3D cell culture models.
Visikol HISTO Buffer Kit Reference: HSB-BK The Visikol HISTO Buffer Kit comes with all of the buffers required to complete the Visikol HISTO approach: 200 mL of Visikol HISTO Washing Buffer 10X, 100 mL of Visikol HISTO Penetration Buffer, 100 mL of Visikol HISTO Blocking Buffer, 100 mL of Visikol HISTO Antibody Buffer, 100 mL of Visikol HISTO Permeabilization Buffer
Visikol HISTO Antibody Buffer Reference: HSK-AB-30 The Visikol HISTO Antibody Buffer is used with immunolabeling in the Visikol HISTO tissue labeling approach. This solution is comprised of PBS/0.2% Tween 20/Heparin/3% donkey serum/5% DMSO.
Visikol HISTO Antibody Buffer Reference: HSK-AB-100 The Visikol HISTO Antibody Buffer is used with immunolabeling in the Visikol HISTO tissue labeling approach. This solution is comprised of PBS/0.2% Tween 20/Heparin/3% donkey serum/5% DMSO.
Visikol HISTO Blocking Buffer Reference: HSK-BB-30 The Visikol HISTO Blocking Buffer is used during the Visikol HISTO immunolabeling process. This solution is comprised of PBS/0.2% Tween™20/6% donkey serum/10% DMSO.
Visikol HISTO Blocking Buffer Reference: HSK-BB-100 The Visikol HISTO Blocking Buffer is used during the Visikol HISTO immunolabeling process. This solution is comprised of PBS/0.2% Tween™20/6% donkey serum/10% DMSO.
Visikol HISTO Penetration Buffer Reference: HSK-PB-30 Visikol HISTO Penetration Buffer is used with the Visikol HISTO immunolabeling process and is comprised of PBS / 0.2% Tween™20 / 0.3 M glycine / 20% DMSO.
Visikol HISTO Penetration Buffer Reference: HSK-PB-100 Visikol HISTO Penetration Buffer is used with the Visikol HISTO immunolabeling process and is comprised of PBS / 0.2% Tween™20 / 0.3 M glycine / 20% DMSO.
Visikol HISTO Permeabilization Buffer Reference: HSK-PMB-30 It is recommend that if you are trying to use Visikol HISTO-1 and Visikol HISTO-2 with larger, more collagenous, and difficult to clear tissues (e.g. liver tissue, archived human tissue, etc.) that you incubate overnight in the Visikol HISTO Tissue Permeabilization Buffer.
Visikol HISTO Permeabilization Buffer Reference: HSK-PMB-100 It is recommend that if you are trying to use Visikol HISTO-1 and Visikol HISTO-2 with larger, more collagenous, and difficult to clear tissues (e.g. liver tissue, archived human tissue, etc.) that you incubate overnight in the Visikol HISTO Tissue Permeabilization Buffer.
Visikol HISTO Washing Buffer 10X Reference: HSK-WB-70 The Visikol HISTO Washing Buffer 10X is used for the Visikol HISTO immunolabeling process. This solution is a concentrate and is comprised of: PPBS with 0.2% Tween-20 and 10µg/mL heparin. This solution should be diluted to 1X concentration with H2O prior to use.
Visikol HISTO Washing Buffer 10X Reference: HSK-WB-200 The Visikol HISTO Washing Buffer 10X is used for the Visikol HISTO immunolabeling process. This solution is a concentrate and is comprised of: PPBS with 0.2% Tween-20 and 10µg/mL heparin. This solution should be diluted to 1X concentration with H2O prior to use.