AP Enhancer Reference: GTX20671 Alkaline phosphatase, an enzyme derived from bovine intestinal mucosa, is often used as a label for in situ hybridization, immunohistochemistry, Southern and Northern hybridization, and DNA sequencing. Detection of this enzyme requires the generation of an insoluble colored reaction end product. The stronger the signal, the better the staining. This is a new stable solution to increase the signal generated by alkaline phosphatase several fold. A single treatment of tissue sections with enhancer before the treatment with substrate/chromogen enables the visualization of difficult to localize antigens. This enhancer works well with Fast Red but can be used with BCIP/NBT and BCIP/INT also. Specimens stained with the above substrates/chromogens can not be dehydrated in ethanol and hence should be mounted in aqueous based mounting medium.
Peroxidase conjugate stabilizer Reference: GTX20673 Peroxidase is one of the most widely used enzyme markers in the immunoassay systems. Because of the higher titre of labeled enzymes, diluted reagent has a tendency to start losing its activity after a few weeks, even at 4ºC. Peroxidase-conjugate diluent is specially designed to solve this problem. Labeled peroxidase when diluted in this diluent will not lose its activity. This diluent will stabilize the peroxidase conjugate and will increase its shelf life for several years when stored at 4ºC. This Peroxidase diluent/stabilizer contains immunologically inert protein to further stabilize the protein peroxidase conjugate.
DAB Enhancer Reference: GTX20675 DAB is a widely used chromogen for immunoperoxidase staining and immunoblotting. It has been well accepted amongst pathologists because of its superior performance as compared to amino ethylcarbazole (AEC). DAB gives cleaner background as opposed to AEC. Specimens stained in DAB can be dehydrated in ethanol and cleared in xylene and can be mounted for permanent record keeping. Strength of signal generated during the immunostaining is the key to a good staining. This is a stable liquid solution which increases the staining intensity of the tissue specimens stained with DAB several fold and hence increases the efficiency of detection systems.
Antigen Retrieval Solution Reference: GTX20970 Trypsin is a commonly used digestive enzyme. In formalin-fixed paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining. This product used to be called ""Carezyme Trypsin Enzymatic Antigen Retrieval Solution"".
AP chromogen BCIP/NBT (ready-to-use) Reference: GTX27468 Alkaline Phosphatase based immunostaining systems are gaining wide acceptance among pathologists because of their relatively high signal to noise ratio. 5-bromo,4-chloro,3-indolylphosphate (BCIP)/nitroblue tetrazolium (NBT) substrate for alkaline phosphatase-based detection systems, is a commonly used substrate chromogen. BCIP acts as the substrate for alkaline phosphatase, and the NBT enhances the purplish-brown final color of the precipitates at the reaction sites.
Antigen retrieval condition-Pepsin (ready to use) Reference: GTX28194 Most fixatives immobilize the tissue antigens by cross linkages. Sometimes, extensive cross linking of proteins in high concentrations can also lead to the masking or destruction of antigens, which leads to poor staining or no staining at all. This loss of staining can be restored to a certain extent by pretreatment of tissue sections with proteolytic enzymes. The unmasking of tissue sections is an important requirement for the optimal staining of antigens like immunoglobulins, keratins, carcinoembryonic antigen, factor VIII related antigen, testosterone, and to a lesser extent S1 protein.
Clear Mount mounting medium-general purpose (Ready-to-use) Reference: GTX28213 Clear-Mount is a water based mounting medium designed for the permanent mounting of hydrated tissues, which may be damaged with organic solvents. Such samples include cell smears, with peroxidase and alkaline phosphatase chromogens.This mounting medium preserves Fast Red, Aminoethylycarbazole (AEC), NBT/BCIP, INT/BCIP chromogens and is also compatible with counter-stain such as Hematoxylin and Nuclear Fast Red. It is also suitable for chromogens like DAB and DAB with Nickel and Cobalt. It is not compatible with H & E staining.
FluoroGel mounting medium Reference: GTX28214 FluoroGel is an aqueous-based mounting medium designed for the preservation of fluorescence-stained tissue sections. It can also be used with conventional fluorescent tracers such as fluorescein (FITC), Rhodamine (TMRITC) and Texas Red. FluoroGel is a non-glycerol based mounting medium. It is specially formulated without glycerol to avoid the deleterious effects of this compound in some of the phycobili-protein based detection systems. This makes FluoroGel the mounting medium of choice when phycobili proteins are used as tracers. This product is not suitable for binary substrates generally used for immunohistochemistry. A product like Clear Mount (aqueous mounting media) or Limonene (organic mounting media) should be used.
Clear Mount mounting medium- general purpose Reference: GTX30703 Clear-Mount is a water based mounting medium designed for the permanent mounting of hydrated tissues, which may be damaged with organic solvents. Such samples include cell smears, with peroxidase and alkaline phosphatase chromogens.This mounting medium preserves Fast Red, Aminoethylycarbazole (AEC), NBT/BCIP, INT/BCIP chromogens and is also compatible with counter-stain such as Hematoxylin and Nuclear Fast Red. It is also suitable for chromogens like DAB and DAB with Nickel and Cobalt. It is not compatible with H & E staining.
Antigen retrieval solution Reference: GTX30709 This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.