Heterophilic antibody blocker for BEK-2226 & BEK-2218 and similar ELISA... Reference: BL-003-1000 BL-003-1000 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing a combination of sheep and mouse immunoreagents. Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-003-1000:BEK-2226, Human proNGF; Application: Serum, Heparin-PlasmaBEK-2218, Human NT4/5; Application: Citrate-Plasma One vial of BL-003-1000 contains 1000 μg IgG which is sufficient as sample diluent additive for one 96-well plate.Other ELISA assays that use sheep and mouse assay antibodies may also benefit from addition of blocker BL-003-1000, but require optimization of working concentration and assay validation for accurate results.
Heterophilic antibody blocker for BEK-2217, BEK-2221, BEK-2237 & BEK-2240 and... Reference: BL-004-500 BL-004-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing sheep immunoreagents. Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-004-500:BEK-2217/2240, Human proBDNF; Application: Serum, Citrate-PlasmaBEK-2221, Human NT3; Application: Plasma (Citrate and EDTA)BEK-2237, Human proBDNF; Application: Serum, EDTA-PlasmaOne vial of BL-004-500 contains 500 μg IgG which is sufficient as sample diluent additive for one 96-well plate.Other ELISA assays that use sheep assay antibodies may also benefit from addition of blocker BL-004-500, but require optimization of working concentration and assay validation for accurate results.
Heterophilic antibody blocker for BEK-2212 and similar ELISA assays-500 µg Reference: BL-005-500 BL-005-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing mouse immunoreagents. Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-005-500:BEK-2212, Human NGF; Application: Citrate-PlasmaOne vial of BL-005-500 contains 500 μg IgG which is sufficient as sample diluent additive for one 96-well plate.Other ELISA assays that use mouse assay antibodies may also benefit from addition of blocker BL-005-500, but require optimization of working concentration and assay validation for accurate results.
Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2-IN-1), Purified... Reference: PE-1608-1 Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2-IN-1), Purified Small Molecule, suitable to Block.
Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2-IN-1), Purified... Reference: PE-1608-10 Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2-IN-1), Purified Small Molecule, suitable to Block.
Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2-IN-1), Purified... Reference: PE-1608-5 Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2-IN-1), Purified Small Molecule, suitable to Block.
Black-Gold II Myelin Ready-to-Dilute Staining Kit with Toluidine Blue O... Reference: TR-100-BG Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system. The Black Gold II Ready-to-Dilute (RTD) Staining Kit allows you to localise myelin, both individual fibres and tracts, along with the option of co-localising cell bodies via the Toluidine Blue counter stain. Black Gold II labelled myelinated fibres appear nearly black while the Toluidine Blue O labelled cellular Nissl bodies are blue under bright field illumination.Black Gold II can demonstrate and characterise specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, domoic acid, 3-nitropropionic acid, Fluoro-Gold and isoniazid. Black Gold II can also be combined with other histochemical markers including Nissl stains, retrogradely transported fluorescent tracers and fluorescent markers of neuronal degeneration. The advantages associated with the Black-Gold II include high resolution, high contrast, short histochemical processing time, versatility and consistent reproducibility.
Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating... Reference: TR-100-FJ The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.
Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating... Reference: TR-100-FJT The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.
Fluoro-Jade B (FJB) Powder for identifying Degenerating Neurons-30 mg Reference: TR-150-FJB The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. The fluorescent dye Fluoro-Jade B (FJB), like its more purified brother Fluoro-Jade C (FJC), is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration. Fluoro-Jade B differs from FJC in that it is a slightly less refined chemical formulation and thus it does not quite provide the same level of signal to noise or high resolution as FJC. Nonetheless FJB is still widely used and works very well as a marker of degenerating neurons and even glia (see Damjanac M et al., Brain Res. 2007;1128(1):40-9). FJB operates nearly identically in protocol to that of FJC, and Fluoro-Jade B is compatible with several other labeling procedures including immunofluorescent and fluorescent Nissl techniques. Fluoro-Jade B stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade B exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.Note: This product is equivalent to discontinued product AG310 from Merck-Millipore.
Fluoro-Jade C (FJC) Powder for identifying Degenerating Neurons-30 mg Reference: TR-160-FJC The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.Note: This product is equivalent to discontinued product AG325 from Merck-Millipore.
Amylo-Glo RTD Amyloid Plaque Stain Reagent-5 mL Reference: TR-300-AG Amylo-Glo RTD Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120- 126). Using Amylo-Glo results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo RTD is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo fluoresces in the UV channel, double and triple labeling experiments can be performed very easily (see protocol).