Horseradish peroxidase - HRP Reference: 4120H Horseradish Peroxidase (HRP) is derived from the root extracts of the horseradish plant. HRP uses H2O2 to oxidize both organic and inorganic compounds. Antibody conjugates, used in ELISA, membrane & IHC applications, biosensors etc. can be developed when coupling HRP to antibodies. HRP is purified by large scale extraction and purification of horseradish roots by means of ultrafiltration, protein precipitation ion exchange and hydrophobic interaction chromatography, resulting in a highly purified, salt free lyophilized product. In ELISA, the main colorimetric substrate for HRP is TMB (3, 3', 5, 5'-tetramethylbenzidine). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of an appropriate stop solution gives a clear yellow color that absorbs at 450nm.
Streptavidin Reference: 4610C Streptavidin is a protein produced by Streptomyces avidinii and isolated by purification from fermentation broth. The pure, homogeneous protein shows predominantly one single band in SDS PAGE. Streptavidin consists of four identical subunits, each bearing an active binding site for biotin. The neutral isoelectric point (pH 5-6) of streptavidin is in the same area where many biological interactions occur and the high affinity of the biotin-streptavidin interaction results in a very quick reaction with a high signal-to-noise Ratio. Streptavidin is useful in combination with, for instance, biotinylated antibodies. Other applications cover Southern and Western Blotting, purification done by use of immobilized Streptavidin and methodologies for DNA/RNA analysis. Streptavidin is non-glycosylated and does not react unspecifically with endogenous lectins when used in assays on cells and tissues.
Streptavidin Reference: 4610H Streptavidin is a protein produced by Streptomyces avidinii and isolated by purification from fermentation broth. The pure, homogeneous protein shows predominantly one single band in SDS PAGE. Streptavidin consists of four identical subunits, each bearing an active binding site for biotin. The neutral isoelectric point (pH 5-6) of streptavidin is in the same area where many biological interactions occur and the high affinity of the biotin-streptavidin interaction results in a very quick reaction with a high signal-to-noise Ratio. Streptavidin is useful in combination with, for instance, biotinylated antibodies. Other applications cover Southern and Western Blotting, purification done by use of immobilized Streptavidin and methodologies for DNA/RNA analysis. Streptavidin is non-glycosylated and does not react unspecifically with endogenous lectins when used in assays on cells and tissues.
Enhanced PLUS Sheep anti-Human-IgG-HRP Reference: 7010N Enhanced PLUS Sheep anti-Human IgG-HRP is a HRP-conjugate specific to Human IgG and is characterized by an enhanced molar ratio of 80-110 HRP enzymes covalently coupled to each molecule. Enhanced PLUS Sheep anti-Human IgG-HRP enables an up to 4-fold increase in sensitivity in ELISA compared to conventional conjugates and offers pg/fg sensitivity levels. The conjugate offers several advantages when limited amounts of antibody or antigen are available: Signal amplification. Ultrasensitive detection. Faster assay time. Recommended when only minimal amounts of specific antibody or antigen are available
Enhanced PLUS Sheep anti-Mouse-IgG-HRP Reference: 7020N Enhanced PLUS Sheep anti-Mouse IgG-HRP is a HRP-conjugate specific to Mouse IgG and is characterized by an enhanced molar ratio of 80-110 HRP enzymes covalently coupled to a polymer backbone. Enhanced PLUS Sheep anti-Mouse IgG-HRP conjugate enables a 2-3 fold increase in sensitivity in ELISA compared to conventional conjugates and offers pg/fg sensitivity levels. The conjugate offers several advantages when limited amounts of antibody or antigen are available: Signal amplification. Ultrasensitive detection. Faster assay time. Recommended when only minimal amounts of specific antibody or antigen are available
Enhanced PLUS Sheep anti-Rabbit-IgG-HRP Reference: 7030N Enhanced PLUS Goat anti-Rabbit IgG-HRP is a HRP-conjugate specific to Rabbit IgG and is characterized by an enhanced molar ratio of 80-110 HRP enzymes covalently coupled to a polymer backbone. Enhanced PLUS Goat anti-Rabbit IgG-HRP conjugate enables an up to 6- fold increase in sensitivity in ELISA compared to conventional conjugate and offers pg/fg sensitivity levels. The conjugate offers several advantages when limited amounts of antibody or antigen are available: Signal amplification. Ultrasensitive detection. Faster assay time. Recommended when only minimal amounts of specific antibody or antigen is available