Recombinant human Ubiquitin (+1) protein Reference: RP10065LQ Ubiquitin (+1) consists of the first 75 amino acids of ubiquitin, but lacks glycine 76 because of the deletion of two of the three nucleotides that encode glycine 76. The resulting frame shift leads to the addition of 20 amino acids (YADLREDPDRQDHHPGSGAQ) after glycine 75. Ubiquitin (+1) can not support ubiquitination reactions. When expressed at high levels, it could inhibit proteasomal degradation. Ubiquitin (+1) was also found in protein aggregates in patient brains of Alzheimer’s disease and other neurodegenerative diseases.
Recombinant human Ubiquitin (K all R) protein Reference: RP10067LQ Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. In 6xHis-Ub(K0), all lysine residues were substituted by arginines, thereby it can only support monoubiquitination, but not polyubiquitin chains.
Recombinant human Ubiquitin (K11 only) protein Reference: RP10068LQ Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. In Ub(K11 only), all lysine residues but lysine 11 were substituted with arginines, thereby it only supports the formation of lysine 11-linked polyubiquitin chains.
Recombinant human Ubiquitin (K27 only) protein Reference: RP10070LQ Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. In Ub(K27 only), all lysine residues but lysine 27 were substituted with arginines, thereby it only supports the formation of lysine 27-linked polyubiquitin chains.
Recombinant human Ubiquitin (K27R) protein Reference: RP10071LQ Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. In Ub(K27R), lysine 27 was substituted with an arginine, thereby it cannot form lysine 27-linked polyubiquitin chains.
Recombinant human Ubiquitin (K29 only) protein Reference: RP10072LQ Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. In Ub(K29 only), all lysine residues but lysine 29 were substituted with arginines, thereby it only supports the formation of lysine 29-linked polyubiquitin chains.
Recombinant human Ubiquitin (K29R) protein Reference: RP10073LQ Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. In Ub(K29R), lysine 29 was substituted with an arginine, thereby it cannot form lysine 29-linked polyubiquitin chains.
Recombinant human Ubiquitin (K33 only) protein Reference: RP10074LQ Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. In Ub(K33 only), all lysine residues but lysine 33 were substituted with arginines, thereby it only supports the formation of lysine 33-linked polyubiquitin chains.
Recombinant human Ubiquitin (K33R) protein Reference: RP10075LQ Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. In Ub(K33R), lysine 33 was substituted with an arginine, thereby it cannot form lysine 33-linked polyubiquitin chains.
Recombinant human Ubiquitin (K6 only) protein Reference: RP10078LQ Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. In Ub(K6 only), all lysine residues but lysine 6 were substituted with arginines, thereby it only supports the formation of lysine 6-linked polyubiquitin chains.
Recombinant human Ubiquitin (K63 only) protein Reference: RP10079LQ Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. In Ub(K63 only), all lysine residues but lysine 63 were substituted with arginines, thereby it only supports the formation of lysine 63-linked polyubiquitin chains.
Recombinant human Ubiquitin (K63R) protein Reference: RP10080LQ Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. In Ub(K63R), lysine 63 was substituted with an arginine, thereby it cannot form lysine 63-linked polyubiquitin chains.