Linear Polyubiquitin Chain Capture Kit Reference: RP10231K This kit is designed for enrichment of cellular proteins conjugated with linear polyubiquitin chains using GST-NEMO (UBAN). Polyubiquitinated proteins bound on GST-NEMO (UBAN) can be precipitated using glutathione resin and eluted by a buffer containing 10 mM glutathione. Polyubiquitinated proteins can be assayed by immunoblotting or mass spectrometry.
Polyubiquitin Chain Capture Kit Reference: RP10232K This kit is designed for enrichment of polyubiquitinated cellular proteins using GST tagged recombinant proteins that bind lysine 48 and lysine 63 polyubiquitin chains. It can be used to enrich polyubiquitinated proteins in whole cell or tissue lysates. Bound proteins can be precipitated using glutathione resin and eluted by a buffer containing 10 mM glutathione, and then assayed by im-munoblotting or mass spectrometry.
Deubiquitinating Enzyme (DUB) Identification Kit Reference: RP10237K This kit is designed to enrich deubiquitinating enzymes in crude or partially purified cell/tissue extracts, followed by identification using immunoblotting or mass spectrometry.
Caspase-3, 7 Activity Fluorometric Assay Kit Reference: RP10240K Caspase-3, 7 Activity Fluorometric Assay Kit can be used for assaying caspase-3, 7 activities in cell/tissue extracts in a 96-well plate format. Each of the supplied fluorogenic substrate and the pan caspase inhibitor Z-VAD-FMK is sufficient for 250 x 100 μl reactions. The supplied TRAIL-treated cell lysate is used as a positive control, sufficient for 5 X 100 μl reactions.
Ubiquitin aldehyde/Ubal Reference: RP10241DLQ Ub aldehyde is a potent and highly specific inhibitor of cysteine deubiquitinating enzymes including the Ub C-terminal hydrolases (UCHs), the Ub-specific proteases (USPs), the ovarian tumor domains (OTUs) proteases and the Machado-Josephine domain (MJD) proteases. Typical working concentration is 2-5 μM in vitro.
HA-Ub-VME Reference: RP10242D A potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs) that is prepared by chemical synthesis. It is N-terminally tagged with an HA-tag (YPYDVPDYA), which allows for sensitive identification or purification of DUBs since it is specifically recognized by anti-HA antibodies. The HA tag is separated from the Ub N-terminus by two aminohexanoic acid (Ahx) linkers for efficient recognition of the HA tag.
Biotin-Ub-PA Reference: RP10243D Biotin-Ub-PA is a newly developed potent and specific inhibitor of deubiquitinating enzymes (DUBs), which has a N-terminal biotin tag. An aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of the biotin binding entity. This activity probe can be used for activity profiling experiments and determining DUB inhibitor specificity. It has two unique capabilities: it forms a covalent linkage with the active site Cys residue of a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses. Biotin-Ub-PA targets all three major DUB families: UCH, USP and OTU
Cy5-Ub-VME Reference: RP10245D Cy5-Ub-VME is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs), which is labeled on the N-terminus with a Cy5 dye (Cy5, Ex 625-650 nm, Em 670 nm). This ubiquitin-based activity probe can be used for activity profiling experiments and labeling of DUBs with a fluorescent dye (Cy5). Cy5 labelling allows for detection of DUB labeling by in-gel fluorescence.
biotin-SUMO2-VME Reference: RP10246D A potent, irreversible and specific inhibitor of deSUMOylation proteases (SENPs) that cleave SUMO2 conjugates. It is N-terminally tagged with a biotin, which allows for sensitive identification of SENPs using HRP-conjugated streptavidin in immunoblotting assays, or purification of SENPs using streptavidin resin. The biotin tag is separated from SUMO2 by an aminohexanoic acid (Ahx) linker that could increase the reactivity of the biotin moiety. In addition, Cys48 in SUMO2 was mutated to Ser.
Ub-Rhodamine110 Reference: RP10248DLQ Ub-Rhodamine 110 is a quenched, fluorescent substrate for deubiquitinating enzymes. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine110 results in an increase in rhodamine fluorescence at 535 nm (Exc. 485 nm).
Recombinant human Ubiquitin thioesterase ZRANB1 protein Reference: RP10005LQ TRABID is an OUT domain-containing deubiquitinating enzyme that exhibits specificity to K29/K33-linked polyubiquitin chains. 6xHis-GST-TRABID(NZF1) contains the N-terminal NZF1 domain (amino acid 1-33) of TRABID fused at the C-terminal of the 6xHis-GST tag. TRABID(NZF1) specifically binds K29/K33 polyubiquitin chains. This protein can be used to pull down K29 and K33 polyubiquitin chains using Ni-NTA resin or glutathione resin.