Native protein isolated from pooled human serum, 312 AA. MW: 34,35 kDa (calculated without glycosylation). Protein identity confirmed by LC-MS/MS (NCBI no. gi|16418467).
Native protein isolated from pooled human seminal plasma, 118 AA, MW 13,52 kDa (calculated without glycosylation). Protein identity confirmed by LC-MS/MS (NCBI no. gi|4505821).
Native protein isolated from Human Adipose Tissue, 338 AA, MW 38,472 kDa (calculated without glycosylation). Protein identity confirmed by MALDI-TOF/TOF (NCBI no. gi|4757756).
Native protein isolated from Human Breast Milk, 691 AA, MW 76,165 kDa (calculated without glycosylation). Protein identity confirmed by LC-MS/MS (NCBI no. gi|119585171).
Total 360 AA. MW: 41.1 kDa (calculated). UniProtKB acc. No. O14793 (Asn24–Ser375). N-terminal His-tag (6 extra AA) and N-terminal linker (2 extra AA). Protein identity confirmed by LC-MS/MS.
Total 152AA, MW: 16.7 kDa (calculated). N-terminal His-tag including the spacer 43AA (highlighted). The AA sequence of the human myostatin part of the fusion protein is corresponding to the UniProtKB/Swiss-Prot entry...
Total 438 AA, M.w. 51.27kDa (calculated). The AA sequence (AA 1–427) is identical to Swiss-Prot-P10909 (AA 23–449, secreted Human Clusterin). C-terminal Flag-tag, 11 extra AA (highlighted).
Total 132 AA. MW: 14.7 kDa (calculated). UniProtKB acc.no. P15090. 131 AA of recombinant Human AFABP and one extra AA, N-terminal methionin (highlighted).
Total 398 AA. MW: 43.4 kDa (calculated). UniProtKB acc.no. Q9UK79. 397 AA of recombinant Human Herstatin and one extra AA, N-terminal methionin (highlighted).
Total 151 AA. MW: 17 kDa (calculated). UniProtKB acc.no. Q9NPG2. 150 AA of recombinant human Neuroglobin and one extra AA, N-terminal methionin (highlighted).
Total 750 AA. MW: 82.4 kDa (calculated). UniProtKB acc.no. P49747. N-Terminal FLAG-tag, 13 extra AA (highlighted). Protein identity confirmed by LC-MS/MS.
Total 291AA, 33,5 kDa (computed). The AA sequence (14–291) is identical to Swiss-Prot-P25311 (AA18–295 mature Zinc-Alfa-2-Glycoprotein). THIRTEEN extra amino acid were fused with N-terminus (highlighted).
Total 289 AA. Mw: 31.9 kDa (calculated). C-Terminal Flag-tag, 12AA (highlighted). The AA sequence is identical to Swiss-Prot- O76061 (STC2, aa 25–302).
Total 294 AA. MW: 32.7 kDa (calculated). N-terminal His-tag, 14 extra AA (highlighted). The AA sequence is identical to UniProtKB/Swiss-Prot entry Q8WWA0 (AA19–298).
Total 206 AA. MW: 23.9 kDa (calculated). N-terminal His-tag and TEV cleavage site, 28 extra AA (highlighted). UniProtKB acc.no. P80188 (Gln21-Gly198). Protein identity confirmed by LC-MS/MS.
Total 204 AA. MW: 23 kDa (calculated). N-terminal His-tag, 21 extra AA (highlighted). The AA sequence is identical to UniProtKB/Swiss-Prot entry Q5VY30 (AA16–199).
Total 171AA. N-terminal His-tag and the protease cleavage site, 31 extra AA (highlighted). The AA sequence is homologous to the amino acid sequence (105–244) of the Human full-length Adiponectin.