FastPure EndoFree Plasmid Maxi Kit Reference: DC202-01 This kit is suitable for extracting 150-300 ml of overnight cultured bacteria, using an improved SDS-alkaline lysis method to lyse the bacteria, the crude extract is selectively combined with a unique endotoxin scavenger and centrifuged to remove endotoxins, and then The silicon matrix membrane in the centrifugal adsorption column selectively binds the plasmid DNA in the solution under high salt and low pH conditions, and then removes impurities and other bacterial components through the rinsing solution, and finally elution with low salt and high pH The buffer eluted purified plasmid DNA from the silicon matrix membrane. The silicon matrix membrane in the centrifugal adsorption column all adopts imported special adsorption membrane, the adsorption amount difference between the column and the column is very small, and the repeatability is good. There is no need to use toxic phenol, chloroform and other reagents, nor ethanol precipitation. It can quickly extract 0.2-1.5 mg of pure high-copy plasmid DNA with an extraction rate of 80-90%. The unique process formula removes endotoxin, the content of endotoxin is very low (< 0.1 EU/μg DNA), and the cell transfection effect is excellent. It can also be directly used in various molecular biology experiments such as enzyme digestion, PCR, in vitro transcription, transformation, sequencing, and so on.Application: High Yield; Compatible with the extraction of plasmids of different lengths; The content of endotoxin is extremely low, and subsequent transfection level experiments can be done; Good stability
Lysozyme Reference: DE103 Lysozyme (lysozyme) is an alkaline enzyme that can hydrolyze mucopolysaccharides in bacteria. It mainly destroys the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall. The cell wall insoluble mucopolysaccharide is decomposed into soluble glycopeptides, causing the cell wall to rupture and the bacteria to dissolve. Mainly used for the digestion of the cell wall of gram-positive bacteria during the preparation of DNA and RNA.Application: Equipped with optimized high-efficiency buffer; Simple, fast and efficient, suitable for the cell wall digestion of Gram-positive bacteria
FastPure Gel DNA Extraction Mini Kit Reference: DC301-01 This kit uses an optimized buffer system and silica gel column purification technology, which can recover 70 bp-20 kb DNA fragments from various concentrations of TAE or TBE agarose gels, and transfer them to the DNA adsorption column under high salt conditions. Direct centrifugation can specifically adsorb DNA and remove other impurities. In addition, this kit can directly purify DNA fragments from PCR products, enzymatic reaction systems or crude DNA products obtained by various other methods, while removing impurities such as proteins, other organic compounds, inorganic salt ions, and oligonucleotide primers. . It can ensure that the purification work is completed in 10-15 min. The purified DNA can be directly used for molecular biology research such as ligation, transformation, restriction enzyme digestion, in vitro transcription, PCR, sequencing, and microinjection.Application: Simple and fast; One box for dual use; High recovery efficiency; Wide range of recovered DNA fragments
FastPure FFPE DNA Isolation Kit Reference: DC105-01 FastPure FFPE DNA Isolation Kit is intended for isolating genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue, overcoming the inhibitory effect caused by formalin crosslinking. The deparaffinization solution in this kit is safer than xylene. The DNA-specific spin column and the unique buffer system also ensure the high quality of obtained DNA. The whole extraction process takes only 20 min (digestion time not counted). The extracted genomic DNA is of good integrity, high purity, and stable quality and can be used for a wide range of downstream applications, including PCR, qPCR, and library preparation.
FastPure Plasmid Mini Kit Reference: DC201-01 FastPure Plasmid Mini Kit purifies 1 - 5 ml of cultured bacteria and lyses the cells by an optimized SDS-alkaline method. The kit utilizes a silica-based membrane technology in the form of a convenient spin column, and binds plasmid DNA in a highly efficient manner under high-salt and low-pH conditions and largely remove proteins, genomic DNA, RNA, and other impurities. In the end, pure plasmid DNA is eluted from the silica membrane with a lowsalt, high-pH elution buffer. The kit recovers up to 35 µg of high-copy plasmid DNA per isolation procedure.The purified plasmid DNA is ready for use in biological experiments such as enzyme digestion, PCR, sequencing, ligation, transformation, and transfection of common passage cells.
FastPure EndoFree Plasmid Mini Kit Reference: DC203-01 This kit is intended for extraction from 1 - 5 ml of overnight bacterial cultures. It adopts anoptimized alkaline lysis method and features a unique silica membrane adsorption technology and special Buffer ERB and Buffer ERW for specific binding of plasmids and effective removal of endotoxins, proteins, and other impurities. The whole extraction process takes only 25 min. The extracted plasmid DNA contains minimal endotoxin residue (≤0.1 EU/μg) and can be used for the transfection of various types of cells and common experiments such as enzyme digestion, PCR, sequencing, and ligation.
FastPure Microbiome DNA Isolation Kit Reference: DC502-01 This kit is intended for fast isolation and purification of host and microbiome DNA from blood, other biological fluids (alveolar lavage fluid, sputum, cerebrospinal fluid, etc.), swab, bacteria/fungi culture, and animal tissue. The kit combines chemical and mechanical lysis methods and can efficiently lyse bacterial and fungal cells with thick cell walls. The extraction process with this kit is safe, fast, and simple as neither toxic reagents (e.g., phenol and chloroform) nor time-consuming procedures (such as alcohol precipitation and RNase treatment) are required. While ensuring efficiency in nucleic acid extraction, the kit also minimizes the contamination of RNA, proteins, lipids, and other impurities. The isolated DNA is suitable for various downstream applications, including PCR, real-time PCR, metagenomic library preparation, and microarray analysis.
FastPure Viral DNA/RNA Mini Kit Pro Reference: RC323-01 FastPure Viral DNA/RNA Mini Kit Pro is a column extraction kit, which can extract viral DNA/RNA from multiple types of samples. The kit uses a unique lysis system, without high temperature incubation, and can significantly improve the recovery rate of trace nucleic acid with carrier RNA. After specific adsorption on silica gel membrane, viral DNA/RNA can be quickly and efficiently purified. For a single sample, it only takes 12 minutes to finish extraction. And the products can be directly used for downstream related experiments such as reverse transcription, PCR, Real-Time PCR, NGS and Northern blot.